Cahn C 31 Microbalance Manual Transmission

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Ferric orthophosphate (FePO 4) has had limited use as an iron fortificant in ready-to-eat (RTE) cereal because of its variable bioavailability, the mechanism of which is poorly understood. Even though FePO 4 has desirable sensory properties as compared to other affordable iron fortificants, few published studies have well-characterized its physicochemical properties.

Semi-crystalline materials such as FePO 4 have varying degrees of molecular disorder, referred to as amorphous content, which is hypothesized to be an important factor in bioavailability. The objective of this study was to systematically measure the physicochemical factors of particle size, surface area, amorphous content, and solubility underlying the variation in FePO 4 bioavailability.

Five commercial FePO 4 sources and ferrous sulfate were added to individual batches of RTE cereal. The relative bioavailability value (RBV) of each iron source, determined using the AOAC Rat Hemoglobin Repletion Bioassay, ranged from 51% to 99% ( p. Introduction Fortifying foods with inorganic iron remains a challenge. Many forms of inorganic iron can be used to fortify food but few forms provide the desired combination of bioavailability and product stability. Freely soluble iron forms such as ferrous sulfate have the highest bioavailability since iron and must be solubilized in gastric juice before it can be absorbed by the body [,,,]. However, soluble iron is also a pro-oxidant, has a metallic off-taste and reacts with many food components causing negative organoleptic changes and shortened shelf life [,,]. As a result, less soluble and thus less reactive forms of iron are often chosen for food applications.

To be absorbed by the small intestine, some portion of the dietary iron must first dissolve in stomach acid []. The solubilities of the various elemental or compound forms of iron depend on their method of manufacture which, in turn, may result in a wide range of bioavailabilities even for the same form of iron [,,,,]. Concerns were raised by the scientific community at the Monterrey Workshop in 2000 about the efficacy of elemental iron due to its variable and often poor bioavailability reported in the literature []. Nevertheless, elemental iron is the most widely used iron fortificant in cereal and cereal products due to its low cost and fewer stability and organoleptic problems []. Results of the SUSTAIN Task Force on Iron Powders collaborative study found the RBVs of commercial elemental iron powders to vary considerably (RBV 21%–64%, relative to ferrous sulfate, defined as RBV 100%) [,]. Commonly used, affordable forms of iron with excellent bioavailability include for example, ferrous sulfate and ferrous fumarate [,,]. However, these highly bioavailable forms of iron pose sensory and stability issues due to the accompanying increase in iron solubility within the food matrix.

Ferric orthophosphate (FePO 4) is preferred over elemental iron for liquid products, light- colored food, and oxidatively sensitive food applications because of its low density, light color, good stability, and non-metallic flavor. However, historically FePO 4 is thought to have little nutritional value and this has limited its use as a fortificant [,,,]. According to a Mintel Global New Product Database (GNPD) search from January 1996–January 2016, there were 2121 food and beverage products including snack/cereal/energy bars, hot and cold cereals, enriched rice and meal replacement drinks containing FePO 4 marketed globally. This compares to over 18,900 food and beverage products marketed during the same period that were fortified with all types of iron []. There is limited information in the literature about the physicochemical properties of FePO 4 and their influence on bioavailability; the paucity of information preclude its widespread use as a food fortificant. Therefore, the objectives of this study were to characterize the physicochemical properties of five sources of commercially available, food-grade FePO 4 to determine which properties have the greatest influence on in vivo relative bioavailability (RBV).